![change nucleotide numbering in gene construction kit change nucleotide numbering in gene construction kit](https://www.genome.gov/sites/default/files/inline-images/Epigenomics_Fact-sheet2020.jpg)
The genetic study of the LOX-1 gene may provide further Negatively charged phospholipids and apoptotic\aged cells as Of the neck domain that connects the transmembrane domainĪnd the carbohydrate-recognition domain (CRD). Unlike human andīovine LOX-1, mouse and rat LOX-1 had a triple-repeat structure To date, cDNAs for bovine, human, rat and Organs, but also in intact endothelium in io and atheromatous intima. LOX-1 wasĮxpressed not only in cultured endothelial cells and vascular-rich LOX-1 is a type-II membrane protein whichīelongs structurally to the C-type lectin family. Recently, weĬloned cDNA for the major endothelial OxLDL receptor, lectinlike OxLDL receptor-1 (LOX-1), from cultured bovine endothelial cells. Macrophages have been identified, the endothelial OxLDL receptor has not been molecularly identified. Īlthough several kinds of scavenger receptor expressed in
![change nucleotide numbering in gene construction kit change nucleotide numbering in gene construction kit](https://static-01.hindawi.com/articles/atox/volume-2014/984319/figures/984319.fig.001.jpg)
Suggests that oxidized low-density lipoprotein (OxLDL) plays aĬritical role in the changes in endothelial function. Smooth-muscle cells in endothelial cells. The enhanced expression of chemoattractant molecules andĪdhesion molecules for leucocytes, and of growth factors for TheseĬhanges include the impairment of the release of nitric oxide, and LOX-1 gene and its choromosomal location.įunctional changes of endothelial cells are implicated in theĮarliest stage of the pathogenesis of atherosclerosis. Here we describe the structural organization of the Since the locus for a familial hypertension hasīeen mapped to the overlapping region, LOX-1 might be the Was a single-copy gene and assigned to the p12.3–p13.2 region ofĬhromosome 12. Similar to the case of other C-type lectin genes. (cytoplasmic, transmembrane and neck domains), and the residual 3 exons encoded the carbohydrate-recognition domain The first 3 exonsĬorresponde d to the different functional domains of the protein Most of the promoterĪctivity of the LOX-1 gene was ascribed to the region (k150 to To be 5 bp downstream from the major site. Of the TATA box and 61 nucleotides upstream from the The major transcriptioninitiation site was found to be located 29 nucleotides downstream GATA-2 binding element, c-ets-1 binding element, 12-O-tetradecanoylphorbol 13-acetate-responsive element and shear-stressresponsive elements, which may mediate the endothelium-specificĪnd inducible expression of LOX-1. The 5h-regulatory regionĬontained several potential cis-regulatory elements, such as Here, we determined the organization of the human LOX-1 gene, Receptor-1 (LOX-1), which is postulated to be involved inĮndothelial dysfunction and the pathogenesis of atherosclerosis. We have reported the cDNA cloning of a modified low-densitylipoprotein (LDL) receptor, designated lectin-like oxidized LDL Medical College, Tokyo, Japan, and RChromosome Research Unit, Faculty of Science, Hokkaido University, Sapporo, Japan Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan, ♭epartment of Pediatric Cardiology, Heart Institute of Japan, Tokyo Women’s Gifu, Japan, ‡Department of Bioscience, National Cardiovascular Center Research Institute, Suita, Osaka 565–8565, Japan, §Department of Molecular Pathophysiology,
![change nucleotide numbering in gene construction kit change nucleotide numbering in gene construction kit](https://www.mdpi.com/ijms/ijms-22-08160/article_deploy/html/images/ijms-22-08160-g006-550.jpg)
*Department of Pharmacology, Faculty of Medicine, Kyoto University, Kyoto 606, Japan, †Second Department of Internal Medicine, Gifu University School of Medicine, Takuma AOYAMA*†, Tatsuya SAWAMURA*‡§, Yoshiyuki FURUTANI¶, Rumiko MATSUOKA¶, Michihiro C. Low-density-lipoprotein receptor-1 (LOX-1) gene Structure and chromosomal assignment of the human lectin-like oxidized